Journal: Cell reports

Article Title: Oxygen level regulates N-terminal translation elongation of selected proteins through deoxyhypusine hydroxylation

doi: 10.1016/j.celrep.2022.110855

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Methionine , Chem Impex , Cat# 00221-100G.

Techniques: Virus, Recombinant, Membrane

Journal: Cell reports

Article Title: Oxygen level regulates N-terminal translation elongation of selected proteins through deoxyhypusine hydroxylation

doi: 10.1016/j.celrep.2022.110855

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Methionine , Chem Impex , Cat# 00221-100G.

Techniques: Virus, Recombinant, Membrane

Journal: Cell reports

Article Title: Oxygen level regulates N-terminal translation elongation of selected proteins through deoxyhypusine hydroxylation

doi: 10.1016/j.celrep.2022.110855

Figure Lengend Snippet:

Article Snippet: Methionine , Chem Impex , Cat# 00221-100G.

Techniques:

Journal: Antibodies

Article Title: Subtractive Immunization as a Method to Develop Respiratory Syncytial Virus (RSV)—Specific Monoclonal Antibodies

doi: 10.3390/antib12040062

Figure Lengend Snippet: Comparison of the neutralizing capacity of ATAC-0025 with that of palivizumab, for different RSV isolates. Neutralization is expressed as the antibody concentration required to neutralize 50% of the RSV-particles present in 1500 PFU/mL RSV dilution. SD = standard deviation.

Article Snippet: The RSV clinical isolates A1998/3-2, A2000/3-4, A2001/3-12 were obtained from BEI resources (Manassas, VA, USA) and the BE-ANT-A12/17, BE-ANT-B2/2017 and BE-ANT-B20/2017 clinical strains were isolated from pediatric patients suffering from an RSV bronchiolitis at the Antwerp university hospital by our own group [ ].

Techniques: Comparison, Neutralization, Concentration Assay

Journal: Clinical chemistry

Article Title: Species Typing of Nontuberculous Mycobacteria using Deoxyribozyme Sensors

doi: 10.1373/clinchem.2018.295212

Figure Lengend Snippet: Sensors were validated using synthetic DNA analytes (1 nM) equivalent to the amplified fragments of NTM 16S rRNA gene.

Article Snippet: We acknowledge Ms. Rachael Rodger of the National Jewish Health Advanced Diagnostic Laboratory and Dr. Stefan Niemann of the National Reference Center for Mycobacteriology (Borstel, Germany) for kindly providing NTM strains and DNA.

Techniques: Amplification

Journal: Applied Microbiology and Biotechnology

Article Title: Comparative genomics of Streptococcus parauberis : new target for molecular identification of serotype III

doi: 10.1007/s00253-020-10683-z

Figure Lengend Snippet: Multiplex PCR products of S. parauberis strains. Lanes: MM, Generuler DNA ladder 1 kb (Fermentas); 1, (subserotype Ia); 2, subserotype Ib; 3, subserotype Ic; 4, serotype II; 5, serotype III; 6 and 7, non-typeable strains; 8, NCDO2020 (serotype IV)

Article Snippet: DNA isolated from pure cultures of the reference strain S. parauberis NCIMB 703043, which belongs to serotype III, was used as a positive control in qPCR reactions.

Techniques: Multiplex Assay

Journal: Applied Microbiology and Biotechnology

Article Title: Comparative genomics of Streptococcus parauberis : new target for molecular identification of serotype III

doi: 10.1007/s00253-020-10683-z

Figure Lengend Snippet: Determination of C q values obtained from the amplification of tenfold dilutions of a purified amplicon of the cps3K gene of the strain NCIMB 703043 obtained by qPCR

Article Snippet: DNA isolated from pure cultures of the reference strain S. parauberis NCIMB 703043, which belongs to serotype III, was used as a positive control in qPCR reactions.

Techniques: Amplification, Purification, Concentration Assay, Intra Assay, Inter Assay

Journal: Applied Microbiology and Biotechnology

Article Title: Comparative genomics of Streptococcus parauberis : new target for molecular identification of serotype III

doi: 10.1007/s00253-020-10683-z

Figure Lengend Snippet: Standard curves obtained by qPCR from the amplification of 10-fold dilutions of purified amplicon obtained using SP3-130F and SP3-130R primers and DNA from the reference strain S. parauberis NCIMB 703043 (black circles) and a clinical isolate of S. parauberis (SK451/04) (gray squares) ( a ) and the corresponding electrophoresis gel showing the band intensities correlating with the number of copies of the cps3K gene ( b ). Lanes: MM, Generuler DNA ladder 1-kb (Fermentas); 1, 2.67 × 10 8 copies; 2, 2.67 × 10 7 copies; 3, 2.67 × 10 6 copies; 4, 2.67 × 10 5 copies; 5, 2.67 × 10 4 copies; 6, 2.67 × 10 3 copies; 7, 2.67 × 10 2 copies

Article Snippet: DNA isolated from pure cultures of the reference strain S. parauberis NCIMB 703043, which belongs to serotype III, was used as a positive control in qPCR reactions.

Techniques: Amplification, Purification, Electrophoresis

Journal: Applied Microbiology and Biotechnology

Article Title: Comparative genomics of Streptococcus parauberis : new target for molecular identification of serotype III

doi: 10.1007/s00253-020-10683-z

Figure Lengend Snippet: Samples tested by qPCR for the detection of S. parauberis serotype III

Article Snippet: DNA isolated from pure cultures of the reference strain S. parauberis NCIMB 703043, which belongs to serotype III, was used as a positive control in qPCR reactions.

Techniques: Concentration Assay